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Timing subdural haematoma
Author(s) -
Mohamed S.,
Rezaie P.,
AlSarraj S.
Publication year - 2002
Publication title -
neuropathology and applied neurobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.538
H-Index - 95
eISSN - 1365-2990
pISSN - 0305-1846
DOI - 10.1046/j.1365-2990.2002.39286_33.x
Subject(s) - stain , medicine , subdural haematoma , pathology , radiology , surgery , staining
One specific technique by which subdural haematoma can be timed has been one of the most challenging problems faced by pathologists. Currently, the most documented technique used for timing trauma is the Perl's stain for haemosiderin indicative of two days time lag between trauma and death. Trauma leads to the activation of a group of cells known as macrophages and offers a possible new means of timing trauma. The aim of this project was to reach a sensible conclusion about timing subdural haematoma by using a variety of markers against macrophages. Materials and methods: Using dural tissue from 28 subjects diagnosed with subdural haematoma, a three step amplification protocol (DAKO, UK) was applied to 5–7 µm sections of the dura, with DAB as chromogen, for subsequent light microscopic analysis. Results: Both qualitative (using a simple scoring system) and quantitative analysis were carried out in this study. Quantitative analysis comprised image capture using a digital camera mounted on a Zeiss axioplan microscope and image analysis by calculation of the percent areas of immunoreactivity per section using Optimas v6.2. Conclusions: PGM 1 and MHC II are not definitive at dating subdural haematoma within the first 48 h period. The early expression of these antibodies makes them good diagnostic tools for dating trauma when combined with other methods such as the Perl's stain.