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Loss of chromosome 10, retinoblastoma and deleted‐in‐colon‐carcinoma (DCC) proteins in glioblastomas
Author(s) -
Hilton D. A.,
Love S.,
Penny M.,
Pobereskin L.
Publication year - 2002
Publication title -
neuropathology and applied neurobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.538
H-Index - 95
eISSN - 1365-2990
pISSN - 0305-1846
DOI - 10.1046/j.1365-2990.2002.39286_22.x
Subject(s) - retinoblastoma , oligodendroglioma , immunohistochemistry , pathology , medicine , chromosome , carcinoma , glioma , biology , cancer research , astrocytoma , genetics , gene
Glioblastomas have a median survival of 9–12 months. However, some patients die within a few weeks of diagnosis and others may survive for 2 years or more. To try to predict survival more accurately, we have evaluated a number of morphological, immunohistochemical and molecular markers in a retrospective series of glioblastomas. Material and methods: Paraffin sections from 107 consecutive glioblastomas diagnosed between 1993 and 1997 were included in the study. All cases were reviewed and the presence of calcification or oligodendroglioma‐like areas noted. Sections were immunostained with commercial antibodies to retinoblastoma and DCC proteins and subjected to in situ hybridization with probes to chromosomes 12 and 10. Results: Loss of retinoblastoma and DCC protein expression was seen in 20% and 71% of glioblastomas respectively. Loss of retinoblastoma protein expression correlated with poor outcome ( P = 0.045). 64% of glioblastomas showed loss of chromosome 10 but this did not have a significant association with survival. The five tumours with oligodendroglioma‐like areas had a median survival of 70 weeks, compared with 27 weeks for those without ( P = 0.014). Conclusion: In this study, age, loss of retinoblastoma protein and a lack of oligodendroglioma‐like areas were predictors of significantly shorter survival in glioblastomas.