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Cellular localization of PDGF mRNAs in developing human forebrain
Author(s) -
Marius Maxwell,
T Galanopoulos,
Janine Neville-Golden,
E. T. HedleyWhyte,
H Antoniades
Publication year - 1998
Publication title -
neuropathology and applied neurobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.538
H-Index - 95
eISSN - 1365-2990
pISSN - 0305-1846
DOI - 10.1046/j.1365-2990.1998.00138.x
Subject(s) - platelet derived growth factor receptor , biology , platelet derived growth factor , microbiology and biotechnology , in situ hybridization , forebrain , receptor , neuroglia , gliogenesis , growth factor , neuroscience , messenger rna , central nervous system , stem cell , neural stem cell , biochemistry , gene
Platelet‐derived growth factor (PDGF) has been implicated in the processes regulating gliogenesis in the CNS. Conflicting in vivo data in rodents have variously implicated either glia or neurons as being the primary source of PDGF. We have used in situ hybridization and immunocytochemical analysis to study the in vivo expression and cellular localization of PDGF‐A, sis/PDGF‐B, together with the two PDGF receptors α and β, in developing human forebrain. In this study we demonstrate the strong expression of mRNA and protein of both PDGF chains, A and B, and their receptors, α and β, in human embryonic glial cells. The neurons, in contrast to glial cells, expressed lower levels of PDGF and PDGF‐receptor mRNAs and protein. Identification of the cell types expressing the PDGF and PDGF‐receptor mRNAs was achieved by counterstaining with antibodies specific for glial cells (GFAP) and neurons (NF). The predominant glial‐specific expression of both PDGF‐A and PDGF‐B, together with the coexpression of their receptors α and β, suggests an important role for the PDGF isoforms in the development of human embryonic glial cells and neurons in vivo .