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Use of green fluorescent protein fusions to analyse the N‐ and C‐terminal signal peptides of GPI‐anchored cell wall proteins in Candida albicans
Author(s) -
Mao Yuxin,
Zhang Zimei,
Wong Brian
Publication year - 2003
Publication title -
molecular microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.857
H-Index - 247
eISSN - 1365-2958
pISSN - 0950-382X
DOI - 10.1046/j.1365-2958.2003.03794.x
Subject(s) - biology , candida albicans , signal peptide , green fluorescent protein , fluorescence , cell wall , terminal (telecommunication) , cell , microbiology and biotechnology , fluorescent protein , signal (programming language) , biochemistry , peptide sequence , gene , physics , quantum mechanics , telecommunications , computer science , programming language
Summary Glycophosphatidylinositol (GPI)‐anchored proteins account for 26–35% of the Candida albicans cell wall . To understand the signals that regulate these proteins’ cell surface localization, green fluorescent protein (GFP) was fused to the N‐ and C‐termini of the C. albicans cell wall proteins (CWPs) Hwp1p, Als3p and Rbt5p. C. albicans expressing all three fusion proteins were fluorescent at the cell surface. GFP was released from membrane fractions by PI‐PLC and from cell walls by β‐glucanase, which implied that GFP was GPI‐anchored to the plasma membrane and then covalently attached to cell wall glucans. Twenty and 25 amino acids, respectively, from the N‐ and C‐termini of Hwp1p were sufficient to target GFP to the cell surface. C‐terminal substitutions that are permitted by the ω rules (G613D, G613N, G613S, G613A, G615S) did not interfere with GFP localization, whereas some non‐permitted substitutions (G613E, G613Q, G613R, G613T and G615Q) caused GFP to accumulate in intracellular ER‐like structures and others (G615C, G613N/G615C and G613D/G615C) did not. These results imply that (i) GFP fusions can be used to analyse the N‐ and C‐terminal signal peptides of GPI‐anchored CWPs, (ii) the ω amino acid in Hwp1p is G613, and (iii) C can function at the ω+2 position in C. albicans GPI‐anchored proteins.