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SetB: an integral membrane protein that affects chromosome segregation in Escherichia coli
Author(s) -
Espeli Olivier,
Nurse Pearl,
Levine Cindy,
Lee Chong,
Marians Kenneth J.
Publication year - 2003
Publication title -
molecular microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.857
H-Index - 247
eISSN - 1365-2958
pISSN - 0950-382X
DOI - 10.1046/j.1365-2958.2003.03736.x
Subject(s) - biology , nucleoid , chromosome segregation , mutant , cell division , mreb , microbiology and biotechnology , integral membrane protein , mutation , genetics , phenotype , escherichia coli , inner membrane , cell cycle protein , membrane protein , chromosome , cell , gene , cell cycle , membrane
Summary setB was identified as a high‐copy suppressor of the partition defect of a mutation in parC , encoding one of the subunits of topoisomerase IV. Deletion of this integral inner membrane protein causes a delay in chromosome segregation, whereas its overproduction causes nucleoid disintegration and stretching, leading to a cell division defect. setB deletion mutants also exhibit a synthetic phenotype when combined with mutations that delete the C‐terminal motor domain of the septal ring protein FtsK. SetB localizes in the cell as a helix and interacts with MreB, the bacterial actin homologue, which also forms a helix. These observations suggest that there may be a link between chromosome segregation and cellular infrastructure.