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Mapping the DNA‐binding domain and target sequences of the Streptomyces peucetius daunorubicin biosynthesis regulatory protein, DnrI
Author(s) -
Sheldon Paul J.,
Busarow Sara B.,
Hutchinson C. Richard
Publication year - 2002
Publication title -
molecular microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.857
H-Index - 247
eISSN - 1365-2958
pISSN - 0950-382X
DOI - 10.1046/j.1365-2958.2002.02886.x
Subject(s) - biology , operon , daunorubicin , genetics , mutagenesis , gene , dna , promoter , streptomyces , dna binding domain , mutant , gene expression , transcription factor , leukemia , bacteria
Summary Streptomyces antibiotic regulatory proteins (SARPs) constitute a novel family of transcriptional activators that control the expression of several diverse anti‐biotic biosynthetic gene clusters. The Streptomyces peucetius DnrI protein, one of only a handful of these proteins yet discovered, controls the biosynthesis of the polyketide antitumour antibiotics daunorubicin and doxorubicin. Recently, comparative analyses have revealed significant similarities among the predicted DNA‐binding domains of the SARPs and the C‐terminal DNA‐binding domain of the OmpR family of regulatory proteins. Using the crystal structure of the OmpR‐binding domain as a template, DnrI was mapped by truncation and site‐directed mutagenesis. Several highly conserved residues within the N‐terminus are crucial for DNA binding and protein function. Tandemly arranged heptameric imperfect repeat sequences are found within the −35 promoter regions of target genes. Substitutions for each nucleotide within the repeats of the dnrG–dpsABCD promoter were performed by site‐directed mutage‐nesis. The mutant promoter fragments were found to have modified binding characteristics in gel mobility shift assays. The spacing between the repeat target sequences is also critical for successful occupation by DnrI and, therefore, competent transcriptional activation of the dnrG–dpsABCD operon.