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Identification and characterization of phage‐resistance genes in temperate lactococcal bacteriophages
Author(s) -
McGrath Stephen,
Fitzgerald Gerald F.,
Sinderen Douwe van
Publication year - 2002
Publication title -
molecular microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.857
H-Index - 247
eISSN - 1365-2958
pISSN - 0950-382X
DOI - 10.1046/j.1365-2958.2002.02763.x
Subject(s) - biology , bacteriophage , plasmid , phagemid , lysogenic cycle , prophage , temperateness , lactococcus lactis , gene , genetics , transduction (biophysics) , lytic cycle , microbiology and biotechnology , virus , escherichia coli , bacteria , lactic acid , biochemistry
Summary The sie 2009 gene, which is situated between the genes encoding the repressor and integrase, on the lysogeny module of the temperate lactococcal bacteriophage Tuc2009, was shown to mediate a phage‐resistance phenotype in Lactococcus lactis against a number of bacteriophages. The Sie 2009 protein is associated with the cell membrane and its expression leaves phage adsorption, transfection and plasmid transformation unaffected, but interferes with plasmid transduction, as well as phage replication. These observations indicate that this resistance is as a result of DNA injection blocking, thus representing a novel superinfection exclusion system. A polymerase chain reaction (PCR)‐based strategy was used to screen a number of lactococcal strains for the presence of other prophage‐encoded phage‐resistance systems. This screening resulted in the identification of two such systems, without homology to sie 2009 , which were shown to mediate a phage‐resistance phenotype similar to that conferred by sie 2009 . To our knowledge, this is the first description of a phage‐encoded superinfection exclusion/injection blocking mechanism in the genus Lactococcus .