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Quantitative analysis of expression of two circadian clock‐controlled gene clusters coding for the bidirectional hydrogenase in the cyanobacterium Synechococcus sp. PCC7942
Author(s) -
Schmitz Oliver,
Boison Gudrun,
Bothe Hermann
Publication year - 2001
Publication title -
molecular microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.857
H-Index - 247
eISSN - 1365-2958
pISSN - 0950-382X
DOI - 10.1046/j.1365-2958.2001.02612.x
Subject(s) - operon , hydrogenase , biology , transcription (linguistics) , promoter , gene , gene expression , synechococcus , regulation of gene expression , protein subunit , enzyme , coding region , biochemistry , genetics , microbiology and biotechnology , cyanobacteria , bacteria , escherichia coli , linguistics , philosophy
Hydrogen metabolism is of central interest in cyanobacterial research because of its potential applications. The gene expression and physiological role of the cyanobacterial bidirectional NAD(P) + ‐reducing hydrogenase are poorly understood. Transcription rates of hoxEF and hoxUYH encoding this enzyme have been studied in Synechococcus sp. PCC7942. P hoxU activity was about three times higher than that of P hoxE . Circadian phasing of both promoters was found to be synchronous and influenced expression levels by at least one order of magnitude. This is the first demonstration of circadian control of gene expression for any hydrogenase. For the majority of P hoxU ‐driven messages, transcription presumably terminates between hoxU and hoxH . Being part of a polycistronic hoxUYHW… operon, hoxW , encoding a protease involved in C‐terminal processing of the hydrogenase large‐subunit HoxH, is mainly expressed by its own promoter, P hoxW . The complex transcript formation may be a key feature for controlling bidirectional hydrogenase expression in vivo .