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The germ tubes of Candida albicans hyphae and pseudohyphae show different patterns of septin ring localization
Author(s) -
Sudbery Peter E.
Publication year - 2001
Publication title -
molecular microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.857
H-Index - 247
eISSN - 1365-2958
pISSN - 0950-382X
DOI - 10.1046/j.1365-2958.2001.02459.x
Subject(s) - septin , hypha , germ tube , biology , mitosis , microbiology and biotechnology , candida albicans , anatomy , botany , cell division , cell , genetics , cytokinesis
The location of the septin ring in the germ tubes of Candida albicans hyphae and pseudohyphae was studied using an antibody to Saccharomyces cerevisiae Cdc11p. In pseudohyphae induced by growth at 35°C in YEPD or Lee's medium, a septin ring formed at or near (mean 1.8 µm) the neck between the mother cell and the germ tube. This became double later in the cycle, and the first mitosis took place across the plane of this double ring. A septin ring also formed at the germ tube neck of developing hyphae induced by serum or growth on Lee's medium at 37°C. However, at later times, this ring became disorganized and disappeared. A second double ring then appeared 10–15 µm (mean 12.5 µm) along the length of the germ tube. The nucleus subsequently migrated out of the mother cell into the germ tube, and the first mitosis took place across the plane of this second septin ring. The relocation of the septin ring in developing hyphae provides a clear‐cut molecular distinction between hyphae and pseudohyphae. Commitment to one type of septin localization and mitosis was shown to occur early in the first mitotic cycle, well before evagination. Germ tubes of hyphae and pseudohyphae also have different widths. A point of commitment to germ tube width was also demonstrated, but occurred later in the cycle, approximately coincident with the time of evagination.