Premium
A novel feature of the multistep phosphorelay in Escherichia coli : a revised model of the RcsC → YojN → RcsB signalling pathway implicated in capsular synthesis and swarming behaviour
Author(s) -
Takeda Shinichiro,
Fujisawa Yojiro,
Matsubara Masahiro,
Aiba Hirofumi,
Mizuno Takeshi
Publication year - 2001
Publication title -
molecular microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.857
H-Index - 247
eISSN - 1365-2958
pISSN - 0950-382X
DOI - 10.1046/j.1365-2958.2001.02393.x
Subject(s) - biology , histidine kinase , protein data bank (rcsb pdb) , response regulator , escherichia coli , enterobacteriaceae , biochemistry , computational biology , histidine , genetics , bacteria , bacterial protein , gene , enzyme
In this study, we re‐investigated the previously characterized RcsC (sensor His‐kinase) → RcsB (response regulator) phosphorelay system that is involved in the regulation of capsular polysaccharide synthesis in Escherichia coli . The previously proposed model hypothesized the occurrence of a direct phosphotransfer from RcsC to RcsB in response to an unknown external stimulus. As judged from the current general view as to the His → Asp phosphorelay, this RcsC → RcsB framework is somewhat puzzling, because RcsC appears to contain both a His‐kinase domain and a receiver domain, but not a histidine (His)‐containing phosphotransmitter domain (e.g. HPt domain). We thus suspected that an as yet unknown mechanism might be underlying in this particular His → Asp phosphorelay system. Here, we provide several lines of in vivo and in vitro evidence that a novel and unique His‐containing phosphotransmitter (named YojN) is essential for this signalling system. A revised model is proposed in which the multistep RcsC → YojN → RcsB phosphorelay is implicated. It was also demonstrated that this complex signalling system is somehow involved in the modulation of a characteristic behaviour of E. coli cells during colony formation on the surface of agar plates, namely swarming.