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Analysis of mRNA decay and rRNA processing in Escherichia coli in the absence of RNase E‐based degradosome assembly
Author(s) -
Ow Maria C.,
Liu Qi,
Kushner Sidney R.
Publication year - 2000
Publication title -
molecular microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.857
H-Index - 247
eISSN - 1365-2958
pISSN - 0950-382X
DOI - 10.1046/j.1365-2958.2000.02186.x
Subject(s) - degradosome , rnase p , biology , messenger rna , rnase h , rnase mrp , rna , escherichia coli , nonsense mediated decay , ribonuclease iii , microbiology and biotechnology , genetics , gene , rna splicing , rna interference
We demonstrate here that the assembly of the RNase E‐based degradosome of Escherichia coli is not required for normal mRNA decay in vivo . In contrast, deletion of the arginine‐rich RNA binding site (ARRBS) from the RNase E protein slightly impairs mRNA decay. When both the degradosome scaffold region and the ARRBS are missing, mRNA decay is dramatically slowed, but 9S rRNA processing is almost normal. An extensive RNase E truncation mutation ( rneδ610 ) had a more pronounced mRNA decay defect at 37°C than the temperature‐sensitive rne‐1 allele at 44°C. Taken together, these data suggest that the inviability associated with inactivation of RNase E is not related to defects in either mRNA decay or rRNA processing.