Premium
Role of flagellins from A and B loci in flagella formation of Halobacterium salinarum
Author(s) -
Tarasov Valery Y.,
Pyatibratov Michael G.,
Tang SenLin,
DyallSmith Michael,
Fedorov Oleg V.
Publication year - 2000
Publication title -
molecular microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.857
H-Index - 247
eISSN - 1365-2958
pISSN - 0950-382X
DOI - 10.1046/j.1365-2958.2000.01677.x
Subject(s) - flagellum , flagellin , halobacterium salinarum , biology , mutant , operon , basal body , gene , microbiology and biotechnology , genetics , archaea
Haloarchaeal flagella are composed of a number of distinct flagellin proteins, specified by genes in two separate operons (A and B). The roles of these flagellins were assessed by studying mutants of H. salinarum with insertions in either the A or the B operon. Cells of the flgA − mutant produced abnormally short, curved flagella that were distributed all over the cell surface. The flgA2 − strain produced straight flagella, mainly found at the poles. The flgB − mutant had flagella of the same size and spiral shape as wild‐type cells, but these cells also showed unusual outgrowths, which appeared to be sacs filled with basal body‐like structures. In broth cultures of this mutant, the medium accumulated flagella with basal body‐like structures at their ends.