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Identification and molecular characterization of a novel Salmonella enteritidis pathogenicity islet encoding an ABC transporter
Author(s) -
Pattery Theresa,
Hernalsteens JeanPierre,
De Greve Henri
Publication year - 1999
Publication title -
molecular microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.857
H-Index - 247
eISSN - 1365-2958
pISSN - 0950-382X
DOI - 10.1046/j.1365-2958.1999.01526.x
Subject(s) - biology , operon , salmonella , complementation , atp binding cassette transporter , mutant , regulon , virulence , bacterial outer membrane , salmonella enteritidis , periplasmic space , gene , genetics , microbiology and biotechnology , transporter , escherichia coli , bacteria
Using a newly constructed minitransposon with a pho A reporter gene in a Salmonella enteritidis pho N mutant, we have identified an iron‐ and pH‐inducible lipoprotein gene sfb A, which is a component of a novel ABC‐type transporter system required for virulence. This gene is located on a 4 kb Salmonella ‐specific chromosomal segment, which constitutes a new pathogenicity islet. This islet encodes an outer membrane protein, OmpX, and contains the operon designated sfb ABC ( Salmonella ferric binding) encoding a putative periplasmic iron‐binding lipoprotein SfbA, a nucleotide‐binding ATPase SfbB and a cytoplasmic permease SfbC, as predicted by their characteristic signature sequences. Inactivation of the sfb A gene resulted in a mutant that is avirulent and induces protective immunity in BALB/c mice. The wild‐type phenotype could be restored by in vivo complementation with the sfb ABC operon. This novel transporter might be involved in iron uptake in Salmonella .