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Alteration of the synthesis of the Clp ATP‐dependent protease affects morphological and physiological differentiation in Streptomyces
Author(s) -
De CrécyLagard Valérie,
ServantMoisson Pascale,
Viala Julie,
Grandvalet Cosette,
Mazodier Philippe
Publication year - 1999
Publication title -
molecular microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.857
H-Index - 247
eISSN - 1365-2958
pISSN - 0950-382X
DOI - 10.1046/j.1365-2958.1999.01364.x
Subject(s) - streptomyces coelicolor , actinorhodin , biology , gene , overproduction , protease , mycelium , genetics , biochemistry , enzyme , mutant , botany
The genes of Streptomyces coelicolor A3(2) encoding catalytic subunits (ClpP) and regulatory subunits (ClpX and ClpC) of the ATP‐dependent protease family Clp were cloned, mapped and characterized. S. coelicolor contains at least two clpP genes, clpP1 and clpP2 , located in tandem upstream from the clpX gene, and at least two unlinked clpC genes. Disruption of the clpP1 gene in S. lividans and S. coelicolor blocks differentiation at the substrate mycelium step. Overexpression of clpP1 and clpP2 accelerates aerial mycelium formation in S. lividans , S. albus and S. coelicolor . Overproduction of ClpX accelerates actinorhodin production in S. coelicolor and activates its production in S. lividans .