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Physical and genetic map of the genome of Vibrio parahaemolyticus : presence of two chromosomes in Vibrio species
Author(s) -
Yamaichi Yoshiharu,
Iida Tetsuya,
Park KwonSam,
Yamamoto Koichiro,
Honda Takeshi
Publication year - 1999
Publication title -
molecular microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.857
H-Index - 247
eISSN - 1365-2958
pISSN - 0950-382X
DOI - 10.1046/j.1365-2958.1999.01296.x
Subject(s) - replicon , biology , vibrio parahaemolyticus , biovar , genomic dna , pulsed field gel electrophoresis , vibrio , vibrio vulnificus , vibrionaceae , genome , genetics , microbiology and biotechnology , southern blot , plasmid , vibrio cholerae , dna , gene , bacteria , genotype
We constructed a physical map of the genomic DNA (5.1 Mb) for Vibrio parahaemolyticus strain AQ4673 by combining 17 adjacent Not I fragments. This map shows two circular replicons of 3.2 and 1.9 Mb. Pulsed‐field gel electrophoresis (PFGE) of undigested genomic DNA revealed two bands of corresponding sizes. Analysis both by Not I digestion and by Southern blot of the two isolated bands confirmed the existence of two replicons. The presence of genes for 16S rRNA on both the replicons indicates that the replicons are chromosomes rather than megaplasmids. The two bands were also seen after PFGE of undigested genomic DNA of V. parahaemolyticus strains other than AQ4673, and of strains belonging to other Vibrio species, such as V. vulnificus, V. fluvialis and various serovars and biovars of V. cholerae . It is noteworthy that V. cholerae O1 strain 569B, a classical biovar, was also shown to have two replicons of 2.9 and 1.2 Mb, which does not agree with a physical map proposed in a previous study. Our results suggest that a two‐replicon structure is common throughout Vibrio species.