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Isolation and characterization of an extracellular haem‐binding protein from Pseudomonas aeruginosa that shares function and sequence similarities with the Serratia marcescens HasA haemophore
Author(s) -
Létoffé Sylvie,
Redeker Virginie,
Wandersman Cécile
Publication year - 1998
Publication title -
molecular microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.857
H-Index - 247
eISSN - 1365-2958
pISSN - 0950-382X
DOI - 10.1046/j.1365-2958.1998.00885.x
Subject(s) - serratia marcescens , biology , bacterial outer membrane , pseudomonas aeruginosa , extracellular , signal peptide , escherichia coli , microbiology and biotechnology , biochemistry , bacteria , function (biology) , peptide sequence , membrane protein , gene , membrane , genetics
The major mechanism by which bacteria acquire free or haemoglobin‐bound haem involves direct binding to specific outer membrane receptors. Serratia marcescens also secretes a haem‐binding protein, HasA, which functions as a haemophore that catches haem and shuttles it to a cell surface specific outer membrane receptor, HasR. We report the isolation and characterization of hasAp , a gene from Pseudomonas aeruginosa. HasAp is an iron‐regulated extracellular haem‐binding protein that shares about 50% identity with HasA. HasAp is required for P. aeruginosa utilization of haemoglobin iron. It can replace HasA for HasR‐dependent haemoblobin acquisition in a system reconstituted in Escherichia coli. HasAp, like HasA, lacks a signal peptide and is secreted by an ABC transporter. These findings show that haemophore‐dependent haem acquisition is not unique to S. marcescens .