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Two independent type III secretion mechanisms for YopE in Yersinia enterocolitica
Author(s) -
Cheng Luisa W.,
Anderson Deborah M.,
Schneewind Olaf
Publication year - 1997
Publication title -
molecular microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.857
H-Index - 247
eISSN - 1365-2958
pISSN - 0950-382X
DOI - 10.1046/j.1365-2958.1997.3831750.x
Subject(s) - secretion , effector , biology , yersinia enterocolitica , signal peptide , secretory protein , cytoplasm , yersinia , microbiology and biotechnology , protein sorting signals , peptide sequence , bacteria , biochemistry , genetics , gene
Pathogenic Yersinia species escape the infected host’s defense mechanisms by targeting cytotoxic Yop proteins into the cytoplasm of macrophages via a type III secretion pathway. Two separate secretion signals contained in YopE were identified, each of which were sufficient but not necessary for the secretion of reporter molecules. One signal is located within the coding sequence of the first 15 amino acids and is sufficient for the secretion of fusion proteins but not required for YopE secretion. The second signal is located downstream at residues 15–100 of YopE and is only recognized by the type III machinery when it is bound to SycE. We propose the existence of two independent mechanisms that allow for the secretion of Yop proteins.