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Double‐glycine‐type leader peptides direct secretion of bacteriocins by ABC transporters: colicin V secretion in Lactococcus lactis
Author(s) -
Belkum Marco J.,
Worobo Randy W.,
Stiles Michael E.
Publication year - 1997
Publication title -
molecular microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.857
H-Index - 247
eISSN - 1365-2958
pISSN - 0950-382X
DOI - 10.1046/j.1365-2958.1997.3111677.x
Subject(s) - lactococcus lactis , biology , secretion , bacteriocin , atp binding cassette transporter , glycine , transporter , colicin , microbiology and biotechnology , biochemistry , bacteria , amino acid , escherichia coli , genetics , gene , lactic acid , antimicrobial
Summary Many non‐lantibiotic bacteriocins of lactic acid bacteria are produced as precursors which have N‐terminal leader peptides that share similarities in amino acid sequence and contain a conserved processing site of two glycine residues in positions ‐1 and ‐2. A dedicated ATP‐binding cassette (ABC) transporter is responsible for the proteolytic cleavage of the leader peptides and subsequent translocation of the bacteriocins across the cytoplasmic membrane. To investigate the role that these leader peptides play in the recognition of the precursor by the ABC transporters, the leader peptides of leucocin A, lactococcin A or colicin V were fused to divergicin A, a bacteriocin from Carnobacterlum divergens that is secreted via the cell's general secretion pathway. Production of divergicin was monitored when these fusion constructs were introduced into Leuconostoc gelidum, Lactococcus lactis and Escherichia coli , which carry the secretion apparatus for leucocin A, lactococcins A and B, and colicin V, respectively. The different leader peptides directed the production of divergicin in the homologous hosts. In some cases production of divergicin was also observed when the leader peptides were used in heterologous hosts. For ABC‐transporter‐dependent secretion in E. coli the outer membrane protein TolC was required. Using this strategy, colicin V was produced in L. lactis by fusing this bacteriocin behind the leader peptide of leucocin A.