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The CIT3 gene of Saccharomyces cerevisiae encodes a second mitochondrial isoform of citrate synthase
Author(s) -
Jia Y.K.,
Bécam A.M.,
Herbert C. J.
Publication year - 1997
Publication title -
molecular microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.857
H-Index - 247
eISSN - 1365-2958
pISSN - 0950-382X
DOI - 10.1046/j.1365-2958.1997.3011669.x
Subject(s) - citrate synthase , biology , complementation , gene isoform , saccharomyces cerevisiae , gene , atp synthase , atp citrate lyase , biochemistry , microbiology and biotechnology , mitochondrion , respiratory chain , enzyme , phenotype
We have identified a third citrate synthase gene in Saccharomyces cerevisiae which we have called CIT3 Complementation of a citrate synthase‐deficient strain of Escherichia coli by lacZ :: CIT3 gene fusions demonstrated that the CIT3 gene encodes an active citrate synthase. The CIT3 gene seems to be regulated in the same way as CIT1 , which encodes the mitochondrial isoform of citrate synthase. Deletion of the CIT3 gene in a Δ cit1 background severely reduced growth on the respiratory substrate glycerol, whilst multiple copies of the CIT3 gene in a Δ cit1 background significantly improved growth on acetate. In vitro import experiments showed that cit3p is transported into the mitochondria. Taken together, these data show that the CIT3 gene encodes a second mitochondrial isoform of citrate synthase.