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Role of stoichiometry between mRNA, translation factor SeIB and selenocysteyl‐tRNA in selenoprotein synthesis
Author(s) -
Tormay P.,
Sawers A.,
Böck A.
Publication year - 1996
Publication title -
molecular microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.857
H-Index - 247
eISSN - 1365-2958
pISSN - 0950-382X
DOI - 10.1046/j.1365-2958.1996.881450.x
Subject(s) - selenocysteine , biology , selenoprotein , transfer rna , translation (biology) , overproduction , biochemistry , messenger rna , genetics , microbiology and biotechnology , gene , rna , enzyme , glutathione , glutathione peroxidase , cysteine
Summary The specialized translation factor SeIB forms a quaternary complex in vitro with selenocysteyl‐tRNA Sec , the selenoprotein mRNA and guanine nucleotides. To gain information on whether this complex is required for selenocysteine insertion in vivo we have studied the effect of unbalanced ratios of the individual components of the complex on UGA readthrough. It was found that overproduction of SeIB in an otherwise wild‐type genetic background reduced UGA read‐through to less than 1 %. Concomitant overexpression of seIC (the gene for selenocysteine‐specific tRNA Sec ) completely reversed the inhibition. Truncation of SeIB from the C‐terminal end abolished function as a translation factor but the truncated molecules, when overproduced, were still able to suppress UGA read‐through. The inhibition was also reversed by overproduction of tRNA Sec . The most plausible explanation is that overproduction of SeIB impairs the statistics of formation of the quaternary complex and that the C‐terminally truncated molecules are still able to bind selenocysteyl‐tRNA Sec and remove it from the pool. The mRNA‐binding capacity, therefore, is physically separated from the selenocysteyl‐tRNA‐binding domain.