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Antagonistic involvement of FIS and H‐NS proteins in the transcriptional control of hns expression
Author(s) -
Falconi Maurizio,
Brandi Anna,
La Teana Anna,
Gualerzi Claudio O.,
Pon Cynthia L.
Publication year - 1996
Publication title -
molecular microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.857
H-Index - 247
eISSN - 1365-2958
pISSN - 0950-382X
DOI - 10.1046/j.1365-2958.1996.436961.x
Subject(s) - biology , footprinting , promoter , microbiology and biotechnology , transcriptional regulation , binding site , transcription factor , dna , dna footprinting , transcription (linguistics) , dna binding protein , dna binding site , gene expression , gene , genetics , linguistics , philosophy
Gel shift and DNase I footprinting experiments showed that Escherichia coli FIS (factor for inversion stimulation) protein binds to at least seven sites in the promoter region of hns . These sites extend from −282 to +25 with two sites, closely flanking the DNA bend located at −150 from the transcriptional startpoint, partly overlapping the H‐NS binding sites involved in the transcriptional autorepression of hns . The interplay between FIS, H‐NS and the hns promoter region were studied by examining the effects of FIS and H‐NS on in vitro transcription of hns–cat fusions, as well as looking at the effect of FIS on preformed complexes containing H‐NS and a DNA fragment derived from the hns promoter region. Taken together, our data suggest that in the cell, FIS and H‐NS interact with the promoter region of hns and influence their respective interactions (possibly competing for the same binding site), eliciting antagonistic effects so that an interplay between these proteins might contribute to the transcriptional control of hns