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Characterization of cpsF and its product CMP‐ N ‐acetylneuraminic acid synthetase, a group B streptococcal enzyme that can function in K1 capsular polysaccharide biosynthesis in Escherichia coli
Author(s) -
Haft Rachel F.,
Wessels Michael R.,
Mebane Mary Fisk,
Conaty Neil,
Rubens Craig E.
Publication year - 1996
Publication title -
molecular microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.857
H-Index - 247
eISSN - 1365-2958
pISSN - 0950-382X
DOI - 10.1046/j.1365-2958.1996.395931.x
Subject(s) - biology , escherichia coli , biochemistry , enzyme , microbiology and biotechnology , biosynthesis , n acetylneuraminic acid , bacterial capsule , polysaccharide , function (biology) , streptococcus , bacteria , sialic acid , genetics , virulence , gene
Group B Streptococcus (GBS) is the foremost cause of neonatal sepsis and meningitis in the United States. A major virulence factor for GBS is its capsular polysaccharide, a high molecular weight polymer of branched oligosaccharide subunits. N ‐acetylneuraminic acid (Neu5Ac or sialic acid), at the end of the polysaccharide side chains, is critical to the virulence function of the capsular polysaccharide. Neu5Ac must be activated by CMP‐Neu5Ac synthetase before it is incorporated into the polymer. We showed previously that a transposon mutant of a serotype III GBS strain which had no detectable capsular Neu5Ac was deficient in CMP‐Neu5Ac‐synthetase activity (Wessels et al ., 1992). In this paper, we report the identification and characterization of cpsF , a gene interrupted by transposon insertion in the previously described Neu5Ac‐deficient mutant. The predicted amino acid sequence of the cpsF gene product shares 57% similarity and 37% identity with CMP‐Neu5Ac synthetase encoded by the Escherichia coli K1 gene, neuA . The enzymatic function of the protein encoded by cpsF was established by cloning the gene in E. coli under the control of the T7 polymerase/promoter. Lysates of E. coli in which the cpsF gene product was expressed, catalysed the condensation of CTP with Neu5Ac to form CMP‐Neu5Ac. In addition, when a CMP‐Neu5Ac synthetase‐deficient mutant of E. coli K1 was transformed with cpsF , K1 antigen expression was restored. We conclude that cpsF encodes CMP‐Neu5Ac synthetase in type III GBS, and that the GBS enzyme can function in the capsule‐synthesis of a heterologous bacterial species.