Premium
The unstable region of Streptomyces ambofaciens includes 210 kb terminal inverted repeats flanking the extremities of the linear chromosomal DNA
Author(s) -
Leblond Pierre,
Fischer Gilles,
Francou FrançoisXavier,
Berger Franca,
Guérineau Michel,
Decaris Bernard
Publication year - 1996
Publication title -
molecular microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.857
H-Index - 247
eISSN - 1365-2958
pISSN - 0950-382X
DOI - 10.1046/j.1365-2958.1996.366894.x
Subject(s) - biology , inverted repeat , genetics , chromosome , restriction enzyme , restriction map , streptomyces coelicolor , genomic dna , microbiology and biotechnology , dna , restriction fragment , gene , streptomyces , genome , nucleic acid sequence , bacteria
Physical maps of the chromosomes of three strains of Streptomyces ambofaciens were constructed by ordering Ase I fragments generated from the genomic DNA as a single linear chromosome of about 8 Mb. The physical maps of the three strains were very similar. For strain DSM40697, a Dra I map was obtained by positioning the Dra I sites relative to the Ase I map. Eighteen genetic markers as well as the deletable and amplifiable region were assigned to the Ase I and Dra I fragments in this strain. The resulting genetic map resembled that of Streptomyces coelicolor A3(2). The twoterminal Ase I fragments exhibited retarded pulsed‐field gel electrophoresis mobility, demonstrating that proteins are covalently bound at this position. A restriction map of this region was made using four additional endonucleases. Repeated sequences present at both ends of the chromosome were mapped as long terminal inverted repeats stretching over 210 kb. This corresponds to the longest terminal inverted repeats so far characterized. The deletable region of S. ambofaciens was localized at the chromosomal extremities.