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Cytoplasmic composition in Pinus densata and population establishment of the diploid hybrid pine
Author(s) -
Song BaoHua,
Wang XiaoQuan,
Wang XiaoRu,
Ding KaiYu,
Hong DeYuan
Publication year - 2003
Publication title -
molecular ecology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.619
H-Index - 225
eISSN - 1365-294X
pISSN - 0962-1083
DOI - 10.1046/j.1365-294x.2003.01962.x
Subject(s) - biology , pinus <genus> , ploidy , population , composition (language) , evolutionary biology , botany , ecology , genetics , gene , demography , sociology , linguistics , philosophy
Sequence and restriction site analyses of the paternally inherited chloroplast rbc L gene and maternally inherited mitochondrial nad 1 fragments from the same set of populations and individuals were used to investigate cytoplasmic composition and population establishment of Pinus densata , a diploid pine that originated through hybridization between P . tabuliformis and P . yunnanensis . Two variable sites and three chlorotypes (TT, TC and GC) were detected on the rbc L gene of the three pines. P . densata harboured the three chlorotypes, two of which (TT, GC) were characteristic of the parental species, respectively. The third chlorotype (TC) was distributed extensively in seven of the 10 P . densata populations analysed, and might represent a mutation type or have been derived from an extinct parent. The distribution of chlorotypes, together with that of mitotypes, indicated that significant founder effect and backcross happened during the population establishment of the hybrid pine. P . tabuliformis and P . yunnanensis had acted as both mother and father donors, i.e. bi‐directional gene flow existed between the two parental species in the past. Population differentiation of P . densata is high, as detected from the cytoplasmic genomes: G ST = 0.533 for cpDNA and G ST = 0.905 for mtDNA. The differences in cytoplasmic composition among the hybrid populations suggest that the local populations have undergone different evolutionary histories.