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Maternal inheritance of a chloroplast microsatellite marker in controlled hybrids between Fraxinus excelsior and Fraxinus angustifolia
Author(s) -
MorandPrieur M. E.,
Vedel F.,
Raquin C.,
Brachet S.,
Sihachakr D.,
FrascariaLacoste N.
Publication year - 2002
Publication title -
molecular ecology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.619
H-Index - 225
eISSN - 1365-294X
pISSN - 0962-1083
DOI - 10.1046/j.1365-294x.2002.01453.x
Subject(s) - biology , chloroplast dna , microsatellite , fraxinus , hybrid , restriction fragment length polymorphism , oleaceae , genetics , introgression , locus (genetics) , genetic marker , sympatric speciation , botany , extranuclear inheritance , polymerase chain reaction , chloroplast , allele , mitochondrial dna , gene
Restriction fragment length polymorphism, polymerase chain reaction–restriction fragment length polymorphism and simple sequence repeat (SSR or microsatellites) analyses were performed to detect chloroplast DNA polymorphisms between two ash species , Fraxinus excelsior and F. angustifolia . Only one SSR locus was found to be polymorphic, confirming the very close relatedness of these species. Inheritance of this marker was studied in hybrids obtained from controlled crosses between the two tree species. Results indicated, for the first time in Oleaceae , that chloroplasts are maternally inherited. This chloroplast SSR marker is now used concomitantly with nuclear markers to analyse ash populations in sympatric areas.