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Phylogenetic divergence in leatherside chub ( Gila copei ) inferred from mitochondrial cytochrome b sequences
Author(s) -
Johnson Jerald B.,
Jordan Steve
Publication year - 2000
Publication title -
molecular ecology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.619
H-Index - 225
eISSN - 1365-294X
pISSN - 0962-1083
DOI - 10.1046/j.1365-294x.2000.00960.x
Subject(s) - biology , clade , phylogenetic tree , cytochrome b , mitochondrial dna , evolutionary biology , phylogenetics , maximum parsimony , zoology , genetics , gene
We examined intra‐specific phylogenetic relationships in leatherside chub, Gila copei . The complete mitochondrial (mt) cytochrome b gene (1140 bp) was sequenced for 30 individuals from 10 populations that span the geographical distribution of this species. Traditional phylogenetic analyses revealed two deeply divergent and evolutionarily distinct mtDNA clades that are geographically separated in northern and southern drainage basins. Inter‐population sequence variation between clades ranged from 7.7 to 8.1%. The northern clade was genetically more similar and phylogenetically more closely related to the selected out‐group Lepidomeda m. mollispinus than to the southern clade, suggesting that the taxonomy of this species may require revision. Sequence variation among populations within clades ranged from 0 to 0.3% in the north and from 0 to 0.7% in the south. Statistical parsimony was used to construct phylogenetic networks of haplotypes within clades. Nested clade analysis revealed that geographical fragmentation has played an important role in genetic structuring within northern and southern clades.