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Amplification of oak DNA from ancient and modern wood
Author(s) -
DumolinLapègue S.,
Pemonge M.H.,
Gielly L.,
Taberlet P.,
Petit R. J.
Publication year - 1999
Publication title -
molecular ecology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.619
H-Index - 225
eISSN - 1365-294X
pISSN - 0962-1083
DOI - 10.1046/j.1365-294x.1999.00788.x
Subject(s) - biology , microsatellite , polymerase chain reaction , chloroplast dna , genetics , dna sequencing , dna , ancient dna , evolutionary biology , gene , genome , allele , population , demography , sociology
A polymorphic noncoding region of chloroplast DNA (cpDNA) was successfully amplified by the polymerase chain reaction (PCR) from various oak wood samples, including recent and more ancient (about 600‐years‐old) samples from different oak species. Adaptation of DNA isolation and amplification protocols was necessary to obtain this result. Polymorphisms useful to distinguish species or geographical origin of these samples could be scored through sequencing. These polymorphisms include one substitution and two microsatellite‐type polymorphisms, due to a variable number of A/T repeats. Identical results were obtained independently in two separate laboratories.