Premium
Amplifying dolphin mitochondrial DNA from faecal plumes
Author(s) -
Parsons Kim M.,
Dallas John F.,
Claridge Diane E.,
Durban John W.,
Balcomb Iii Kenneth C.,
Thompson Paul M.,
Noble Les R.
Publication year - 1999
Publication title -
molecular ecology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.619
H-Index - 225
eISSN - 1365-294X
pISSN - 0962-1083
DOI - 10.1046/j.1365-294x.1999.00723-8.x
Subject(s) - marsh , george (robot) , biology , archaeology , environmental ethics , library science , zoology , ecology , geography , history , art history , philosophy , wetland , computer science
© 1999 Blackwell Science Ltd, Molecular Ecology, 8, 1753–1768 for paternity analyses. Six loci that did not amplify reliably but may be useful with different primers are (repeat array followed by GenBank Accession no.): (GA)13 (AF143977); (TG)13 (AF143978); (CT)19 (AF143981); (TG)11 (AF143983); (GA)24 (AF143984); and (CT)23(AT)12 (AF143985). The five loci used for paternity yielded 77 alleles with a mean of 15 alleles per locus. Observed and expected heterozygosities ranged from 0.49 to 0.94. Hardy–Weinberg equilibrium was tested with Fisher’s exact test of genepop version 3.1d (Raymond & Rousset 1995). A significant excess of homozygotes was observed in three populations for DE48 and in five populations for DE54 (Table 1), presumably the result of null alleles. The expected exclusion probabilities for single loci ranged from 0.23 to 0.87 with a multilocus expectation of > 0.995 (Marshall et al. 1998). Together, these loci provided enough variation to resolve paternity for a large portion of the D. excelsa seed population.