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Direct conjugal transfers of Ti plasmid to soil microflora
Author(s) -
TeyssierCuvelle S.,
Mougel C.,
Nesme X.
Publication year - 1999
Publication title -
molecular ecology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.619
H-Index - 225
eISSN - 1365-294X
pISSN - 0962-1083
DOI - 10.1046/j.1365-294x.1999.00689.x
Subject(s) - plasmid , biology , agrobacterium , ti plasmid , transformation (genetics) , microbiology and biotechnology , bacteria , rhizobiaceae , gene , genetics , symbiosis
The bacterial species in soil that can receive a Ti plasmid by conjugation from Agrobacterium spp. were investigated. In order to have direct access to the potential reservoir of Ti plasmid amongst soil microflora, the conjugal system consisting of a multiply auxotrophic derivative of C58 (ST‐96‐4) and a derivative of pTiC58Δ accR (pSTiEGK) containing a triple antibiotic‐resistance cassette in traM was used to transfer the Ti plasmid in a complex soil microflora used as the recipient. Numerous transconjugants were obtained by this method but none was identified as Agrobacterium . This could be explained by the low density of Agrobacterium in the tested soil. As indicated by analysis of the ribosomal gene rrs , transconjugants recovered directly from soil were found to be new bacterial species which appeared to be closely related to Sinorhizobium spp.