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The use of amplified fragment length polymorphism (AFLP) in the isolation of sex‐specific markers
Author(s) -
Griffiths RICHARD,
Orr KATE
Publication year - 1999
Publication title -
molecular ecology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.619
H-Index - 225
eISSN - 1365-294X
pISSN - 0962-1083
DOI - 10.1046/j.1365-294x.1999.00578.x
Subject(s) - amplified fragment length polymorphism , biology , genetics , isolation (microbiology) , fragment (logic) , polymorphism (computer science) , restriction fragment length polymorphism , evolutionary biology , genotype , gene , bioinformatics , population , genetic diversity , demography , computer science , programming language , sociology
Sex identification is a problem in research and conservation. It can often be solved using a DNA test but this is only an option if a sex‐specific marker is available. Such markers can be identified using the amplified fragment length polymorphism (AFLP) technique. This is usually a taxonomic method, as it produces a DNA fingerprint of 50–100 PCR bands. However, if male and female AFLP products are compared, sex‐specific markers are confined to the heterogametic sex and can rapidly be identified. Once a marker is found, AFLP can be used to sex organisms directly or the marker can be sequenced and a standard PCR test designed.