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Random amplified polymorphic DNA (RAPD) markers reveal less genetic variation in the endangered plant Cerastium fischerianum var. molle than in the widespread conspecific C . fischerianum var. fischerianum (Caryophyllaceae)
Author(s) -
Maki M.,
Horie S.
Publication year - 1999
Publication title -
molecular ecology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.619
H-Index - 225
eISSN - 1365-294X
pISSN - 0962-1083
DOI - 10.1046/j.1365-294x.1999.00517.x
Subject(s) - rapd , biology , selfing , analysis of molecular variance , genetic diversity , endangered species , genetic variation , population , genetic variability , botany , genetics , genetic structure , ecology , gene , genotype , habitat , demography , sociology
Random amplified polymorphic DNA (RAPD) analysis was used to assess the level of genetic diversity in an endangered plant Cerastium fischerianum var. molle and the widespread conspecific C . fischerianum var. fischerianum . At both the variety and the population level, C . fischerianum var. molle had a lower level of RAPD variation than var. fischerianum . Analysis of molecular variance ( AMOVA ) showed that most of the genetic diversity exists among populations for both varieties. Genetic differentiation among populations probably resulted from the highly selfing habits of both varieties. To conserve C . fischerianum var. molle genetically, preservation of only a part of the population is insufficient.