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A study of genetic diversity in Populus nigra subsp. betulifolia in the Upper Severn area of the UK using AFLP markers
Author(s) -
WINFIELD M. O.,
ARNOLD G. M.,
COOPER F.,
LE RAY M.,
WHITE J.,
KARP A.,
EDWARDS K.J.
Publication year - 1998
Publication title -
molecular ecology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.619
H-Index - 225
eISSN - 1365-294X
pISSN - 0962-1083
DOI - 10.1046/j.1365-294x.1998.00299.x
Subject(s) - amplified fragment length polymorphism , biology , genetic diversity , principal component analysis , restriction fragment length polymorphism , polymorphism (computer science) , similarity (geometry) , botany , evolutionary biology , genetics , genotype , population , statistics , demography , sociology , mathematics , artificial intelligence , computer science , image (mathematics) , gene
A survey of amplified fragment length polymorphism (AFLP) was conducted to examine genetic diversity in 146 individuals of British, native black poplar ( Populus nigra subsp. betulifolia ) and three individuals considered by collectors to be non‐ betulifolia poplars. Using two primer pairs, a total of 147 bands were detected of which 82 (56%) were polymorphic in at least one individual. Cluster analysis and principal coordinates analysis of the calculated similarity matrix revealed a low level of genetic diversity, although a loose clustering into five groups could be identified, one of which contained the non‐ betulifolia individuals. Examination of the spatial distribution of the other four groups (all betulifolia ) revealed a general correlation between geographic proximity and genetic similarity. On the basis of the polymorphism observed, it was possible to identify a small number of individual plants which exhibit maximum diversity and might therefore be suitable for inclusion in a replanting programme designed to maintain at least the existing low level of polymorphism observed within British black poplar.