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Development of multiple genetic markers for studies of genetic variation in arbuscular mycorrhizal fungi using AFLP™
Author(s) -
Rosendahl S.,
Taylor J. W.
Publication year - 1997
Publication title -
molecular ecology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.619
H-Index - 225
eISSN - 1365-294X
pISSN - 0962-1083
DOI - 10.1046/j.1365-294x.1997.d01-213.x
Subject(s) - biology , amplified fragment length polymorphism , genetic variation , intraspecific competition , arbuscular mycorrhizal fungi , spore , recombination , interspecific competition , genetic variability , population , genetic marker , genetics , genetic diversity , evolutionary biology , botany , genotype , ecology , gene , horticulture , demography , sociology , inoculation
Intraspecific and interspecific genetic variation was studied among arbuscular mycorrhizal fungi. DNA was extracted from single spores and variation was analysed by AFLP (Amplified Fragment Length Polymorphism). The patterns of amplified fragments revealed substantial genetic variation between spores from the same isolates. Possible recombination in the fungi was studied by comparing the obtained data with data generated by artificial recombination of the data sets. No evidence for recombination was found by the analysis, suggesting that the fungi reproduce clonally. The AFLP technique generated a large number of fragments, and the potential for using the technique in population genetic studies of these important unculturable biotrophic fungi is discussed.