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Analysis of genetic diversity in common loon Gavia immer using RAPD and mitochondrial RFLP techniques
Author(s) -
Dhar A. K.,
Pokras M. A.,
Garcia D. K.,
Evers D. C.,
Gordon Z. J.,
AlcivarWarren A.
Publication year - 1997
Publication title -
molecular ecology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.619
H-Index - 225
eISSN - 1365-294X
pISSN - 0962-1083
DOI - 10.1046/j.1365-294x.1997.00218.x
Subject(s) - rapd , biology , restriction fragment length polymorphism , mitochondrial dna , haplotype , genetic diversity , genetics , cytochrome b , population , gene , polymerase chain reaction , genotype , demography , sociology
We used random amplified polymorphic DNA (RAPD) and restriction fragment length polymorphisms (RFLP) of mitochondrial cytochrome b (cyt b ) gene to evaluate the genetic diversity in common loon Gavia immer populations from two regions in the United States: New England (NE) and Michigan (MI). RAPD analysis with 18 primers showed 74% polymorphism in NE and 50% in MI loons (similarity coefficient F = 0.92). Although no population‐specific markers were found, the frequencies of some RAPD bands varied between the two populations suggesting geographical differences. RFLP analyses with Bam HI enzyme and a 307‐bp mitochondrial cyt b gene showed four haplotypes in the NE loon samples and two in the MI samples. The mtDNA haplotype diversity was 0.74 for NE and 0.51 for MI loons, supporting the RAPD data that NE loons have greater genetic diversity than MI loons.