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A method for extraction and analysis of high quality genomic DNA from ixodid ticks
Author(s) -
Hill C. A.,
Gutierrez J. A.
Publication year - 2003
Publication title -
medical and veterinary entomology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 82
eISSN - 1365-2915
pISSN - 0269-283X
DOI - 10.1046/j.1365-2915.2003.00425.x
Subject(s) - biology , ixodidae , amblyomma americanum , genomic dna , acari , dna extraction , tick , rhipicephalus , dna , restriction enzyme , genetics , zoology , polymerase chain reaction , gene , virology
. Currently, no published methods describe the extraction of high molecular weight genomic DNA from ixodid ticks (Acari: Ixodidae) and commonly used methods of extraction are not well adapted for use with members of this family. A method for extraction of minimally degraded genomic DNA from ixodid ticks that can be completed in one or two days is described. The method produces DNA which is of sufficient size (>24 kb) for use in Southern analysis and which is readily digestible by restriction endonucleases. Southern analysis using a cytochrome P450 gene probe, demonstrates the success of our method with genomic DNA extracted from two species of Ixodidae, the lone star tick, Amblyomma americanum (Linnaeus) and the cattle fever tick, Boophilus microplus (Canestrini).