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Presence of Anopheles culicifacies B in Cambodia established by the PCR‐RFLP assay developed for the identification of Anopheles minimus species A and C and four related species
Author(s) -
VAN BORTEL W.,
SOCHANTA T.,
HARBACH R. E.,
SOCHEAT D.,
ROELANTS P.,
BACKELJAU T.,
COOSEMANS M.
Publication year - 2002
Publication title -
medical and veterinary entomology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 82
eISSN - 1365-2915
pISSN - 0269-283X
DOI - 10.1046/j.1365-2915.2002.00376.x
Subject(s) - biology , sensu , restriction fragment length polymorphism , anopheles culicifacies , vector (molecular biology) , monophyly , species complex , polymerase chain reaction , anopheles , virology , genetics , zoology , malaria , gene , phylogenetics , clade , phylogenetic tree , genus , recombinant dna , immunology
A polymerase chain reaction‐restriction fragment length polymorphism (PCR‐RFLP) assay developed for identification of five species of the Anopheles minimus Theobald group and a related mosquito species of the Myzomyia Series (Diptera: Culicidae) was applied to morphologically identified adult female specimens collected in Ratanakiri Province, north‐eastern Cambodia. In addition to finding An. aconitus Dönitz, An. minimus species A and An. pampanai Büttiker & Beales, some specimens showed a new restriction banding pattern. Siblings of specimens that exhibited this new PCR‐RFLP pattern were morphologically identified as An. culicifacies James sensu lato . Based on nucleotide sequences of the ribonuclear DNA internal transcribed spacer 2 region (ITS2) and the mitochondrial cytochrome oxidase I gene (COI), these specimens were recognized as An. culicifacies species B ( sensu Green & Miles, 1980), the first confirmed record of the An. culicifacies complex from Cambodia. This study shows that the PCR‐RFLP assay can detect species not included in the initial set‐up and is capable of identifying at least seven species of the Myzomyia Series, allowing better definition of those malaria vector and non‐vector anophelines in South‐east Asia.