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DNA in situ hybridization on polytene chromosomes of Simulium sanctipauli at loci relevant to insecticide resistance
Author(s) -
Boakye D. A.,
Cornel A. J.,
Meredith S. E.,
Brakefield P. M.,
Collins F. H.
Publication year - 2000
Publication title -
medical and veterinary entomology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 82
eISSN - 1365-2915
pISSN - 0269-283X
DOI - 10.1046/j.1365-2915.2000.00230.x
Subject(s) - polytene chromosome , biology , simulium , genetics , insecticide resistance , gene , dna , in situ , dna–dna hybridization , in situ hybridization , microbiology and biotechnology , larva , drosophila melanogaster , botany , gene expression , toxicology , physics , meteorology
Summary A DNA technique for in situ hybridization developed by Kumar & Collins (1994) for use on polytene chromosomes of adult Anopheles mosquitoes (Diptera: Culicidae) was modified for use with Simulium larval salivary gland chromosomes (Diptera: Simuliidae). Cloned fragments of several Simulium genes (coding for aspartate amino transferase, cytochrome P450 and DNA polymerase) were successfully mapped physically by assigning specific band locations in Simulim sanctipauli V. & D. This represents the first attempt at locating genes beyond the resolution of linkage to inversions in any blackfly species.