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Genetic differentiation of some Glossina morsitans morsitans populations
Author(s) -
Wohlford D. L.,
Krafsur E. S.,
Griffiths N. T.,
Marquez J. G.,
Baker M. D.
Publication year - 1999
Publication title -
medical and veterinary entomology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 82
eISSN - 1365-2915
pISSN - 0269-283X
DOI - 10.1046/j.1365-2915.1999.00185.x
Subject(s) - biology , fixation index , inbreeding , genetics , glossinidae , genetic diversity , population , allele , allele frequency , hardy–weinberg principle , fixation (population genetics) , population genetics , evolutionary biology , zoology , microsatellite , gene , demography , sociology
Summary To study the population structure of Glossina morsitans morsitans Westwood (Diptera: Glossinidae), polymerase chain reaction (PCR) and single‐strand conformational polymorphism (SSCP) methods were used to estimate mitochondrial DNA diversity at four loci in six natural populations from Zambia, Zimbabwe and Mozambique, and in two laboratory cultures. The Zambian and Zimbabwean samples were from a single fly belt. Four alleles were recorded at 12S and 16S1 , and five alleles at 16S2 and COI . Nucleotide sequencing confirmed their singularities. Chi‐square contingency tests showed that allele frequencies differed significantly among populations. Mean allele diversities in populations averaged over loci varied from 0.14 to 0.61. Little loss in haplotype diversity was detected in the laboratory cultures thereby indicating little inbreeding. Wright’s fixation index F ST in the natural populations was 0.088 ± 0.016, the correlation of haplotypes within populations relative to correlations in the total. A function of its inverse allows an estimate of the mean equivalent number of females exchanged per population per generation, 5.2. No correlation was detected between pairwise genetic distance measures and geographical distances. Drift explains the high degree of differentiation.