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Larvicidal toxicity of Japanese Bacillus thuringiensis against the mosquito Anopheles stephensi
Author(s) -
SAITOH HIROYUKI,
HIGUCHI KAZUHIKO,
MIZUKI EIICHI,
OHBA MICHIO
Publication year - 1998
Publication title -
medical and veterinary entomology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 82
eISSN - 1365-2915
pISSN - 0269-283X
DOI - 10.1046/j.1365-2915.1998.00090.x
Subject(s) - anopheles stephensi , biology , bacillus thuringiensis , serotype , microbiology and biotechnology , indian subcontinent , anopheles , bacillales , veterinary medicine , strain (injury) , larva , malaria , botany , bacteria , aedes aegypti , medicine , history , ancient history , genetics , anatomy , bacillus subtilis , immunology
Japanese isolates of Bacillus thuringiensis were screened for larvicidal activity against the mosquito Anopheles stephensi , the urban malaria vector of the Indian subcontinent. Among more than 30 strains identified, larvicidal activity causing >80% mortality in 72 h was demonstrated for 41/1449 (2.8%) isolates. The majority of strains and isolates (97.2%) exhibited little or no larvicidal activity. Anopheles ‐active strains belonged to more than 12 H serotypes, especially H3ade (serovar fukuokaensis ) and H44 (serovar higo ). SDS‐PAGE profiles of inclusion proteins showed 4 distinct types among 6 active strains examined. The most active Japanese isolates were H20 strain 89‐T‐34‐14 (LC 50 4.4 μg/ml) and H44 serovar higo strain 74‐E‐45‐24 (LC 50 7.6 μg/ml), respectively, 13‐fold and 23‐fold less active than the international standard H14 serovar israelensis (LC 50 0.33 μg/ml).