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Mapping and characterization of B cell linear epitopes in the conservative regions of hepatitis C virus envelope glycoproteins
Author(s) -
Olenina L. V.,
Nikolaeva L. I.,
Sobolev B. N.,
Blokhi. P.,
Archakov A. I.,
Kolesanova E. F.
Publication year - 2002
Publication title -
journal of viral hepatitis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.329
H-Index - 100
eISSN - 1365-2893
pISSN - 1352-0504
DOI - 10.1046/j.1365-2893.2002.00358.x
Subject(s) - epitope , virology , glycoprotein , cd81 , antigen , epitope mapping , virus , hepatitis c virus , biology , hepatitis b virus , immunodominance , microbiology and biotechnology , immunology
Forty‐eight overlapping octapeptides covering highly conservative regions of E1 and E2 hepatitis C virus (HCV) envelope proteins were synthesized and tested by ELISA against different groups of sera obtained from HCV‐infected patients. All sera from patients with acute infection, except a single case of serum reactivity with the region HINRTALN, were nonreactive with any peptide. Sera obtained from chronic patients reacted with 12 peptides from five selected regions. Two immunodominant B epitopes were found, one being the precisely mapped antigenic site RMAWDM positioned inside the earlier shown immunodominant epitope from E1, and the second site, PALSTGLIH from E2, detected for the first time. New minor antigenic site was determined as PTDCFRKH from E2. We found only minor seroreactivity for one of the putative sites involved in CD81 binding, PYCWHYAP.