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Flaviviridae polymerase and RNA replication
Author(s) -
Bartholomeusz A.,
Thompson P.
Publication year - 1999
Publication title -
journal of viral hepatitis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.329
H-Index - 100
eISSN - 1365-2893
pISSN - 1352-0504
DOI - 10.1046/j.1365-2893.1999.00173.x
Subject(s) - rna dependent rna polymerase , polymerase , rna polymerase , biology , ns5b , rna , replicon , rna polymerase i , rna polymerase ii , microbiology and biotechnology , genetics , hepatitis c virus , gene , virus , gene expression , genome , promoter , hepacivirus
Sequence motifs within the non‐structural protein NS5 or NS5B of the members of the family Flaviviridae suggest that this protein is the RNA‐dependent RNA polymerase. This protein has now been expressed in various in vitro systems and used in polymerase assays. To understand the role of the RNA polymerase in RNA replication, this review will examine not only the polymerase protein but also the other proteins in the RNA replication complex. To date, several groups have investigated the interaction of these proteins both in vitro and in vivo and also the interaction of these proteins with the RNA signals at the 3’ terminus of the RNA. Infectious clones and replicons containing the non‐structural proteins have now been generated and these will be useful tools in understanding the processes of initiation and elongation of both positive and negative RNA synthesis.