Premium
Effects of carprofen ( R and S enantiomers and racemate) on the production of IL‐1, IL‐6 and TNF‐α by equine chondrocytes and synoviocytes
Author(s) -
ARMSTRONG S.,
LEES P.
Publication year - 2002
Publication title -
journal of veterinary pharmacology and therapeutics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.527
H-Index - 60
eISSN - 1365-2885
pISSN - 0140-7783
DOI - 10.1046/j.1365-2885.2002.00397.x
Subject(s) - carprofen , lipopolysaccharide , tumor necrosis factor alpha , chemistry , enantiomer , in vivo , pharmacology , interleukin , proinflammatory cytokine , cytokine , endocrinology , medicine , inflammation , biology , biochemistry , stereochemistry , microbiology and biotechnology
Chondrocytes and synoviocytes harvested from the joints of healthy horses were maintained in tissue culture. Production of the cytokines interleukin‐1 (IL‐1), interleukin‐6 (IL‐6) and tumour necrosis factor‐ α (TNF‐ α ) in response to lipopolysaccharide (LPS), and the effects of addition of carprofen (racemate and R and S enantiomers) were determined. Lipopolysaccharide failed to stimulate TNF‐ α activity in both cell types but concentrations of IL‐1 and IL‐6 were both increased in a concentration and time‐related manner. Both carprofen enantiomers and the racemic mixture attenuated the increase in IL‐6 induced by LPS in synoviocytes, and S carprofen exerted a similar effect on chondrocytes. Neither enantiomer nor the racemate of carprofen suppressed the increase in IL‐1 release produced by LPS in chondrocytes and synoviocytes. An action of carprofen to suppress IL‐6 release might contribute to the actions which occur in vivo .