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Measurement of ketoprofen in horse urine using gas chromatography‐mass spectrometry
Author(s) -
Kim J. Y.,
Kim S. J.,
Paeng K.J.,
Chung B. C.
Publication year - 2001
Publication title -
journal of veterinary pharmacology and therapeutics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.527
H-Index - 60
eISSN - 1365-2885
pISSN - 0140-7783
DOI - 10.1046/j.1365-2885.2001.00353.x
Subject(s) - ketoprofen , chromatography , chemistry , urine , detection limit , flurbiprofen , gas chromatography–mass spectrometry , selected ion monitoring , mass spectrometry , correlation coefficient , extraction (chemistry) , pharmacology , medicine , biochemistry , statistics , mathematics
A gas chromatographic‐mass spectrometric (GC‐MS) method for the determination of ketoprofen, a non‐steroidal anti‐inflammatory drug (NSAID), in horse urine by selected ion monitoring (SIM) mode is described. Urine samples (2 mL) were extracted by liquid–liquid extraction with diethyl ether. The residues were then evaporated, derivatized and injected into the GC‐MS system. Validation of the GC‐MS method in the SIM mode using flurbiprofen as the internal standard (IS) included linearity studies (10–10 000 ng/mL), recovery (95%) and limit of quantitation (LOQ) (10 ng/mL). The response was linear, with a correlation coefficient (0.9998) for ketoprofen. When applied to horse urine samples, the present method allowed detection of ketoprofen up to 16 days in FULL SCAN mode after a topical application of 1.1 g of ketoprofen in a gel formulation.