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Cytotoxicity in pig hepatocytes induced by 8‐quinolinol, chloramine‐T and natamycin
Author(s) -
María-Aránzazu Martínez,
Arturo Anadón,
María Luisa FernándezCruz,
María Jesús Villamide Díaz,
Marta Martínez,
María Teresa Frejo,
Maria F. Tafur
Publication year - 2000
Publication title -
journal of veterinary pharmacology and therapeutics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.527
H-Index - 60
eISSN - 1365-2885
pISSN - 0140-7783
DOI - 10.1046/j.1365-2885.2000.00243.x
Subject(s) - natamycin , cytotoxicity , chemistry , neutral red , glutathione , lactate dehydrogenase , chloramine t , mtt assay , cytotoxic t cell , cytosol , biochemistry , microbiology and biotechnology , enzyme , in vitro , biology , food science , organic chemistry
The potential cytotoxic effects of the compounds 8‐quinolinol, chloramine‐T and natamycin have been studied in isolated pig hepatocytes. The relative cytotoxicity of these compounds was evaluated on the basis of the leakage of cytosolic lactate dehydrogenase (LDH), 3‐(4,5 dimethyl)thiazol‐2‐yl,‐2,5‐diphenyl tetrazolium bromide (MTT) reduction by mitochondrial dehydrogenases, uptake of neutral red (NR) by cytosolic lysosomes, glutathion (GSH) depletion and oxidized glutathion (GSSG) efflux after 24 h exposure. Evaluation of the 20%, 50% and 80% reduced absorbance data obtained from the parameters NR 20 , NR 50 , and NR 80 , and MTT 20 , MTT 50 and MTT 80 enabled us to rank these compounds in decreasing order of cytotoxicity: 8‐quinolinol > natamycin > chloramine‐T. Also for the parameters LDH and GSH, chloramine‐T appears to be less cytotoxic than natamycin and 8‐quinolinol. Our study demonstrated that pig hepatocytes may be a useful model for examining cytotoxic events of drugs to be used in pigs, therefore avoiding possible extrapolation problems due to species differences.