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Development and evaluation of an enzyme‐linked immunosorbent assay (ELISA) for the determination of the trypanocidal drug homidium in serum of treated cattle
Author(s) -
GA Murilla,
Mark C Eisler,
A. S. Peregrine,
J. M. Ndung'u,
P.H. Holmes
Publication year - 1999
Publication title -
journal of veterinary pharmacology and therapeutics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.527
H-Index - 60
eISSN - 1365-2885
pISSN - 0140-7783
DOI - 10.1046/j.1365-2885.1999.00216.x
Subject(s) - pharmacology , pharmacokinetics , drug , enzyme , detection limit , chemistry , drug resistance , medicine , biology , chromatography , microbiology and biotechnology , biochemistry
Two enzyme‐linked immunosorbent assays (ELISA) for the determination of homidium in serum of treated cattle have been developed and evaluated. One is a direct competition (Assay 1) and the other an indirect competition assay (Assay 2). Both assays are highly sensitive with a limit of detection of 0.1 ng homidium per mL serum. Homidium levels were measurable in serum of cattle for over 2 months following administration of a single intramuscular (i.m.) dose at 1 mg/kg bodyweight. The level of sensitivity afforded by these assays makes them potentially useful tools in the pharmacokinetic evaluation of homidium and for invetigating drug resistance or causes of drug failure. Assay 2 was chosen as being most suitable for further studies.