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Increased ACTH and corticosterone secretion induced by different methods of paradoxical sleep deprivation
Author(s) -
SUCHECKI DEBORAH,
LOBO LETÍCIA,
HIPÓLIDE DÉBORA,
TUFIK SERGIO
Publication year - 1998
Publication title -
journal of sleep research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.297
H-Index - 117
eISSN - 1365-2869
pISSN - 0962-1105
DOI - 10.1046/j.1365-2869.1998.00122.x
Subject(s) - corticosterone , endocrinology , medicine , sleep deprivation , basal (medicine) , privation , stressor , adrenocorticotropic hormone , hypothalamic–pituitary–adrenal axis , maternal deprivation , biology , circadian rhythm , hormone , neuroscience , insulin
The methods used to induce paradoxical sleep (PS) deprivation are believed to be stressful. In the present study, two methods were compared in regard to their ability to activate the hypothalamic‐pituitary‐adrenal (HPA) axis. Animals were placed on multiple large (MLP) or small (MSP) platforms or on single large (SLP) or small (SSP) platforms and blood sampled at the end of a 4‐day period of PS deprivation (experiment 1) or on Days 1 (short‐term) and 4 (long‐term) of PS deprivation (experiment 2). ACTH and corticosterone (CORT) levels were determined by RIA. The results of experiment 1 showed that all experimental animals presented increased ACTH response, compared to controls. CORT levels, however, were only elevated in MSP animals, suggesting increased adrenal sensitivity. Experiment 2 showed that ACTH levels of MSP animals were higher than MLP and SSP animals, and that animals placed on the multiple platform tanks showed the highest ACTH levels on Day 4 of manipulation. CORT levels were elevated in the animals kept over small platforms, and these levels where higher on Day 1 than basal and further elevated on Day 4 of PS deprivation. These results indicate that the multiple platform technique induces a distinct activation of the HPA axis, and that PS deprivation may act as an additional stressor.