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Impaired Somatodendritic Responses to Pituitary Adenylate Cyclase‐Activating Polypeptide (PACAP) of Supraoptic Neurones in PACAP type I ‐Receptor Deficient Mice
Author(s) -
Jamen F.,
Alonso G.,
Shibuya I.,
Widmer H.,
Vacher C.M.,
Calas A.,
Bockaert J.,
Brabet P.,
Dayanithi G.
Publication year - 2003
Publication title -
journal of neuroendocrinology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.062
H-Index - 116
eISSN - 1365-2826
pISSN - 0953-8194
DOI - 10.1046/j.1365-2826.2003.01075.x
Subject(s) - vasopressin , endocrinology , medicine , supraoptic nucleus , oxytocin , adenylate kinase , neuropeptide , chemistry , receptor , cyclase , pituitary adenylate cyclase activating peptide , hypothalamus , vasopressin receptor , biology , antagonist , vasoactive intestinal peptide
The role of pituitary adenylate cyclase‐activating polypeptide (PACAP) type I receptor (PAC1 receptor) in regulating hypothalamic supraoptic neurones was investigated using PAC1 receptor‐deficient male mice ( PAC1 −/− ). The effects of PACAP on [Ca 2+ ] i were investigated in freshly dissociated supraoptic neurones and on the somatodendritic release of vasopressin and oxytocin, examined on intact supraoptic nuclei. In supraoptic neurones from wild‐type mice ( PAC1 +/+ ), 100 n m PACAP induced an increase in [Ca 2+ ] i and release of vasopressin and oxytocin, whereas in heterozygous ( PAC1 +/− ) and null‐mutant mice ( PAC1 −/− ), PACAP was much less effective. PACAP had no effect on these two parameters when applied to isolated neurohypophysial nerve terminals of PAC1 +/+ and PAC1 −/− mice, and rats. In conclusion, the PAC1 receptor is solely responsible for the PACAP‐induced [Ca 2+ ] i signalling and secretion of vasopressin and oxytocin in the somatodendritic region of supraoptic neurones.