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Effects of Serotonin, GABA and Neuropeptide Y on Seabream Gonadotropin Releasing Hormone Release In Vitro from Preoptic‐Anterior Hypothalamus and Pituitary of Red Seabream, Pagrus major
Author(s) -
Senthilkumaran B.,
Okuzawa K.,
Gen K.,
Kagawa H.
Publication year - 2001
Publication title -
journal of neuroendocrinology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.062
H-Index - 116
eISSN - 1365-2826
pISSN - 0953-8194
DOI - 10.1046/j.1365-2826.2001.00645.x
Subject(s) - medicine , endocrinology , neuropeptide y receptor , hypothalamus , serotonin , biology , gonadotropin releasing hormone , gabaa receptor , preoptic area , neuropeptide , chemistry , receptor , hormone , luteinizing hormone
The effects of serotonin (5‐HT), GABA and neuropeptide Y (NPY) on in vitro release of seabream (sb) gonadotropin releasing hormone (GnRH) from slices of the preoptic‐anterior hypothalamus (P‐AH) and pituitary of red seabream were studied. 5‐HT, GABA and NPY all stimulated the release of sbGnRH from the P‐AH but not from the pituitary of immature red seabream. They also stimulated sbGnRH release from the P‐AH with a similar potency during the course of gonadal development. Specific agonists and/or antagonists of 5‐HT, GABA and NPY showed that 5‐HT and GABA utilize 5‐HT 2 and GABAA receptor subtypes, respectively, to mediate their action, and that NPY employs at least NPY Y1 and NPY Y2 receptor subtypes to stimulate sbGnRH release. Combinations of different antagonists for 5‐HT, GABA and noradrenaline/adrenaline did not block the stimulatory influence of NPY on release of sbGnRH, indicating that the action of NPY on the sbGnRH neuronal system is probably direct.