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Expression of mRNA for Voltage‐Dependent and Inward‐Rectifying K Channels in GH 3 /B 6 Cells and Rat Pituitary
Author(s) -
Wulfsen,
Hauber,
Schiemann,
Christiane K. Bauer,
Schwarz Schwarz
Publication year - 2000
Publication title -
journal of neuroendocrinology
Language(s) - Slovenian
Resource type - Journals
SCImago Journal Rank - 1.062
H-Index - 116
eISSN - 1365-2826
pISSN - 0953-8194
DOI - 10.1046/j.1365-2826.2000.00447.x
Subject(s) - gene isoform , messenger rna , microbiology and biotechnology , chemistry , crystallography , biology , biochemistry , gene
The expression of mRNA for voltage‐dependent (K v ) and inward‐rectifying K channels (K ir ) was studied in clonal rat somato‐mammotroph cells (GH 3 /B 6 cells) and rat pituitary using reverse transcription‐polymerase chain reaction (RT‐PCR). In GH 3 /B 6 cells transcripts for 16 different K v channel α ‐subunits (seven Shaker‐related: K v 1.2, K v 1.4, K v 1.5, K v 2.1, K v 3.2, K v 4.1, K v 5.1; six EAG: eag1, erg1, erg2, elk1–elk3; three KCNQ: KCNQ1–KCNQ3) and for five different K ir channel α ‐subunits (K ir 1.1, K ir 2.3, K ir 3.2, K ir 3.3, K ir 6.2) were found. In addition, transcripts for a short isoform of K v β 2 and transcripts for K v β 3 subunits were present. In rat pituitary transcripts for 21 different K v channel α ‐subunits (11 Shaker‐related: K v 1.3, K v 1.4, K v 1.6, K v 2.1, K v 2.2, K v 3.2, K v 3.4, K v 4.1, K v 4.2, K v 4.3, K v 6.1; seven EAG: eag1, erg1–erg3, elk1–elk3; three KCNQ: KCNQ1–KCNQ3) and nine K ir channel α ‐subunits (K ir 1.1, K ir 2.2, K ir 3.1–K ir 3.4, K ir 4.1, K ir 6.1, K ir 6.2) were found. In addition, all tested auxiliary subunits (K v β 1–K v β 3, minK, SUR1, SUR2) are expressed in the pituitary. The results indicate that the macroscopic K currents in GH 3 /B 6 and pituitary cells are presumably mediated by K channels constructed by a larger number of K channel α ‐subunits and auxiliary β ‐subunits than previously distinguished electrophysiologically and pharmacologically.