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Evidence for Oestrogen Receptor α‐Immunoreactivity in Gonadotrophin‐Releasing Hormone‐Expressing Neurones
Author(s) -
Jonathan Butler,
Maria Sjöberg,
Clive W. Coen
Publication year - 1999
Publication title -
journal of neuroendocrinology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.062
H-Index - 116
eISSN - 1365-2826
pISSN - 0953-8194
DOI - 10.1046/j.1365-2826.1999.00347.x
Subject(s) - medicine , endocrinology , gonadotropin releasing hormone , immunohistochemistry , receptor , preoptic area , hormone , biology , chemistry , luteinizing hormone
Having used the cingulate cortex to demonstrate the validity of our methods for detecting hitherto unrecognized oestrogen receptor alpha (ER α )‐immunoreactive neurones, we have now employed immunoprecipitation and double‐label immunohistochemistry to investigate whether the ER α protein is present in gonadotrophin‐releasing hormone (GnRH)‐containing cells. The immortalized GnRH cell line GT1–7 and GnRH neurones within the rat preoptic area were found to possess ER α ‐immunoreactivity (ER α ‐IR). These observations indicate that oestrogen may regulate the synthesis and release of GnRH by direct actions on GnRH neurones.